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Preclinical protocols reported in the literature document typical concentrations of 101000 nM in B16-F10 and MNT-1 melanocyte models for studying the cAMP/PKA/MITF/tyrosinase cascade

Ruhnau KJ, Meissner HP, Finn JR, et al

In human metabolic research 3 , elevated IGF-1 levels correlated with improved muscle energy utilization and reduced fatigue during recovery phases

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Samples were vortexed again, centrifuged (16,000 g , 10 min, 4 C) and then all supernatant was removed to leave a protein pellet