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This problem is remedied by using a Methyl B12 supplement

During the first 72 hours , swelling is usually at its peak

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This has been my only change so I know its working

The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

Where do you inject the B12