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doi: 10.1016/j.jhep.2015.11.004 15 ChalasaniNYounossiZLavineJECharltonMCusiKRinellaMet al

Supernatant was discarded and pellets were incubated in Ammonium-Chloride-Potassium buffer (ACK) (0.15 mM NH 4 Cl, 10 mM KHCO 3 and 0.5 M EDTA, pH 7.3) for 5 min at RT and centrifuged at 500 g for 5 min at 4 C

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Gender-specific data were available in some studies, reporting 268 males and 288 females