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Coimmunoprecipitation (co-IP) and mass spectrometry For the immunoprecipitation (IP) assay, HK1 cells transfected with the empty vector or Flag-GPX4 expression plasmid were lysed in IP lysis buffer

Retatrutides numbers come from an earlier, smaller phase 2 study, while tirzepatide and semaglutide were measured in large phase 3 trials over longer periods

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Mixing them degrades potency and stability

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