After 18 hours, cells were labelled with 10 nM JFX646 Halo ligand (kind gift from the Lavis lab, Janelia) in optiMEM (Gibco) for 3 minutes, followed by 3 washes in mKRB buffer (140 mM NaCl, 2.8 mM KCl, 2 mM MgCl 2 , 1 mM CaCl 2 , 10 mM HEPES, 10 mM glucose, pH 7.4) at 37 C
Beyond collagen, GHK-Cus effects on the extracellular matrix extend to elastin (30% increase), decorin (302% increase at 1 nanomolar concentration), glycosaminoglycans, dermatan sulfate, and chondroitin sulfate
20 , e1022e1039 (2022)
Space meals and snacks so they match your injection schedule and energy needs
Side effects are more likely to occur during dose increases
Nausea Vomiting Constipation Diarrhea Abdominal pain Stomach discomfort These effects are usually dose-dependent and tend to improve as your body adjusts to the medication