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After treatment with the blocking solution (0.3% Triton-X and 3% BSA in 0.01 M PBS) to block non-specific staining and increase permeability to antibodies, the brain sections were incubated with anti-c-Fos rabbit polyclonal antibodies (abcam) in the blocking solution at 4 C overnight

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doi: 10.1210/er.2011-1052 14 DuringMJCaoLZuzgaDSFrancisSJFitzsimonsHLJiaoXet al

First-generation GLP-1 receptor agonists necessitated once or twice-daily dosing, resulting in substantial peak-to-trough variations that caused inconsistent glycaemic regulation and heightened gastrointestinal adverse effects (Nauck and Meier 2019)