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For cell culture, use sterile PBS

For example, DAMPs such as extracellular histones can trigger inflammation and senescence in vascular smooth muscle cells by activating the NLRP3 inflammasome and the AMP-activated protein kinase (AMPK)-forkhead box O4 (FOXO4) pathway, thus exacerbating histone-induced damage in mice

Rhonda Patrick: I mean I think the most obvious would be vitamin D, which is actually, as you know, a steroid hormone that we produce when we're in the sun, depending on the time of year we can make it in our skin

doi: 10.3389/fpls.2018.00751 Received 15 February 2018 Accepted 16 May 2018 Published 07 June 2018 Volume 9 - 2018 Edited by Kendal Hirschi, Baylor College of Medicine, United States Reviewed by Toshiro Shigaki, The University of Tokyo, Japan

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