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The powder was suspended in 1 ml nuclear isolation buffer (NIB

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No high-quality clinical trial has demonstrated that B12 injections produce meaningful weight loss independent of dietary and exercise changes

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In contrast, rats treated with metformin did not display this aberrant enrichment in Galactose metabolism and Primary bile acid biosynthesis, indicating that metformin may exert a regulatory effect on these specific metabolic pathways, potentially aiding in the restoration of normal metabolic function or at least mitigating metabolic dysregulation