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The Chinese journal of physiology

A virus expressing a Cre-dependent neural activating DREADD [AAV2-hSyn-DIO-hM3D(Gq)-mCherry] was infused bilaterally into the VTA (6.00 mm A/P, 0.50 mm M/L, and 8.50 mm D/V) in a volume of 500 l over 90 s (titer = 1.0 10 12 gc/ml)

The goal is minimal agitation throughout the process

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