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However, persistent burning lasting more than 30 minutes, spreading redness beyond 1 inch of the injection site, swelling that worsens after 24 hours, or warmth accompanied by fever suggests possible infection or allergic reaction requiring medical evaluation
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Moreover, mice with systemic knockout of SLC25A39 (including the heart) exhibited severe anemia, a complete lack of red blood cells, and iron overload [62]
Use a circular motion, starting from the center of the injection site and moving outward