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A gradient of 240% of solvent B (0.1% (v/v) FA in acetonitrile) was run at 250 nL/min for 150 min, and the eluted peptides were injected into an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific) run at Top Speed (max cycle time 3 s) in data-dependent acquisition mode

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Examples include polyethylene glycol (MiraLAX) and stool softeners, such as docusate sodium (Colace)

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