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The bacteriostatic agent inhibits microbial growth, extending the usability of reconstituted peptide solutions under proper refrigeration conditions

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Thereafter, cells were washed once and incubated for 30 min at 37 with HBSS buffer (pH 7.4) supplemented with 0.1% BSA and 10 mM HEPES

Probiotic support potentially enhances this regulatory optimization, though direct evidence for the combination remains limited

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