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GIP works synergistically with GLP-1 rather than simply adding to its effects

5001000 ng total RNA was treated with DNase1 (Thermo Fisher Scientific, EN0521), and cDNA was synthesized using random hexamers and SuperScript III (Thermo Fisher Scientific, 1808044), followed by qPCR for gene expression or regular PCR for amplification of the full-length Glp1r transcript

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Leucine supplementation does not enhance acute strength or running performance but affects serum amino acid concentration

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Reference: FOXO4-DRI induces keloid senescent fibroblast apoptosis by promoting nuclear exclusion of upregulated p53-serine 15 phosphorylation