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At the L4 stage, a total of around 100 F1 worms per condition were transferred to RNAi bacteria-seeded plates containing 10 M 5-FU to prevent the growth of progeny

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Rathore AS, et al

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Nature 637 , 622630 (2025)

However, injectable applications require consideration of total copper load, particularly in patients with conditions affecting copper metabolism such as Wilsons disease or Menkes disease