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Cell culture and treatments The murine macrophage cell line RAW 264.7 (TIB-71, American Type Culture Collection, Manassas, VA, USA) was cultured at 37C and under 5% CO 2 condition in Dulbeccos modified Eagles medium (DMEM) containing 4.5 g/L glucose, 10% fetal bovine serum (FBS), and 1% penicillinstreptomycin (P/S) mixture

Insulin is responsible for removing glucose (sugar) from the bloodstream and transporting it to the cells for energy

She demonstrated strong clinical knowledge, clearly explained treatment options, dosing considerations, expected timelines, and safety protocols, and ensured informed consent before proceeding

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Center for Drug Evaluation and Research