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K., & Demirci, B

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Following the third wash, 10% of the bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards on a rotator shaker for 10 min at 4 C

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but mTORC1 is inactivated when glutamine production is triggered

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Neither, however, offers the prolonged exposure of a modified GHRH analog such as CJC-1295, which is engineered for extended activity

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Samples are then added into these wells and bind to the immobilized (capture) antibody

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