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In addition, this method has also been used in biomarker research for diseases such as diabetes and obesity
Markowska M et al
It is synthesised in the cytosol by glutamate-cysteine ligase (EC 6.3.2.2) and glutathione synthetase, with the first, rate-limiting step feedback-inhibited by glutathione itself and intracellular concentrations typically 1 to 8 mM (Griffith, Free Radical Biology and Medicine, 1999)
The possible mechanisms are as follows
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