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These methodological changes would be critical to testing our hypothesis given the coefficient of variation for Glx and NAA across ALS and control groups was as high as 34% in some studies, as shown in Table 5, although the average coefficient of variation was on the order of 1015% which is consistent with current state of the art methodology at 3 T (64)

10.1053/j.gastro.2015.09.039 47 KoppelmannT.PollakY.MogilnerJ.BejarJ.CoranA

PLoS ONE 9 (7), e101267 (2014)

Bouhrara, M

BPC-157 remains categorized strictly as an unapproved research compound reserved for laboratory evaluation

The pellets were washed with PBS buffer (pH 7.2) 4-5 times and then mixed with 5 loading buffer