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For GHK-Cu pretreatment, mice received intraperitoneal injections of GHK-Cu of 1 or 10 g/g every 24 hours for three days prior to LPS administration

Proper sterile handling is essential to avoid infection or tissue irritation

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Anyone using diabetes medicines should avoid changing treatment without professional guidance

This approach reflects general CHB populations with heterogeneous drinking levels rather than alcohol-selected subgroups

Each method has advantages and limitations