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The diagnostic algorithm was subsequently developed according to the diagnostic values obtained

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However, the disappearance of GSH was not accounted for by its conversion to GSSG as this form was not detected in the representative HPLC chromatogram shown

Independent replication of some findings strengthens the evidence base

Using genome mining, NcEgt2, a pyridoxal phosphate (PLP)-binding cysteine desulfurase enzyme that converts hercynylcysteine sulfoxide to EGT, was identified and the C-S lyase activity of Egt2 (NCU11365) to generate EGT was reconstituted in vitro (31) (Fig