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Ryan et al., 2020) used C57BL/6 mice to construct a high-fat diet (HFD)-induced diabetes model followed by metformin treatment

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Seek urgent medical attention for severe persistent abdominal pain, persistent vomiting, blood in stools, or signs of dehydration

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However, long-term use may increase the risk of adverse effects, particularly non-motor symptoms such as hallucinations and sleep disturbances

Concomitant diseases of patients in this trial included, but were not limited to, heart failure (12%), history of ischemic stroke (8%) and history of a MI (36%)