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FEBS Lett 584:889897 Krylova VV, Dubrovo NP, Izmailov SF (2007) The effect of metabolites on the pH gradient and membrane potential of the bean peribacteroid membrane

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These models, derived from discarded human skin, maintain some of the structural and functional complexity of native skin

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One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)