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Since most people sleep 68 hours after an evening injection, early nausea (which typically peaks 14 hours post-injection) often resolves during sleep, reducing daytime discomfort

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Moreover, expression levels of neuronal differentiation 1 ( NEUROD1 ), which is a transcription factor with a critical role in cell fate and maturation (47), follow similar patterns to the aforementioned genes, with the highest expression in the -4 subcluster (Figure 3D)

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Of note, oxidative stress induces lipid impairment primarily affecting cell membrane lipids such as phospholipids and cholesterol, thereby significantly impacting the permeability and fluidity of the lipid bilayer [47]

Earlier administration (2-3 hours) tends to produce gradual sleep pressure that builds naturally