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12 hours post-transduction, cells were labelled with SNAP-Surface Alexa Fluor 647, washed 1x in PBS and imaged in RPMI-1640 media without phenol red using a Nikon Eclipse Ti microscope with an ORCA-Flash 4.0 camera (Hamamatsu) and Metamorph software (Molecular Devices) with a 20x air objective and 488 and 647 nm lasers