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The neuronal pathological changes were also observed in the acquired digital images saved as uncompressed 24-bit RGB TIFF files in the software program AnalySIS (Olympus Soft Imaging System GmbH, Mnster, Germany) performing quantitative analysis of neuronal damage in the karyopyknotic areas

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Hepatic fibrosis is a hallmark of NASH pathogenesis 41 , and NASH mice consistently displayed stable fibrosis