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Once the bacteria had been heat-shocked, they we plated on plates containing kanamycin, IPTG and x-gal

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Another study with CuCl 2 treated Hep G2 cell line demonstrated that mitochondrial toxicity is an intermediate-level event associated with copper toxicity to Hep G2 cells while lysosomal damage is the early event and plasma damage a late feature associated with copper toxicity 34