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Sci Rep 16 , 23643 (2026)
IGF-1 LR3's structural modifications allow it to remain unbound and biologically active for 2030 hours post-injection, delivering sustained systemic exposure to IGF-1 receptor signaling
coli strain XL1-Blue and purified by affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl and 50 mM sodium phosphate, pH 8.0 (refs 11, 12, 18, 33, 53)
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Patel A, Cholkar K, Agrahari V, Mitra AK
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