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Kraljevi Paveli S, Simovi Medica J, Gumbarevi D, Filoevi A, Prulj N, Paveli K

5540% solution B in 45 min, followed by re-equilibration of the column with 90% solution B for 1 min

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d 5 -JA was added to the extraction solvent (isopropanol: formic acid at 99.5: 0.5, v/v) as internal standard