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Dessa antas vara associerade med den ospecifika bindningen till flera olika subtyper av melanokortinreceptorer

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Drops (2 L) consisting of a 1:1 ratio of C20A mutant OsDHAR protein (21 mg/mL in 20 mM Tris-HCl, pH 8.0 and 150 mM NaCl) and crystallization solution (100 mM Bis-Tris HCl, pH 6.16.8, 28% (w/v) PEG 3350 and 10 mM CaCl 2 ) were equilibrated against 0.5 mL of the same solution

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160 l of the supernatant was transferred to another set of tubes and dried using a nitrogen evaporator