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The S-Traps containing trapped protein were washed twice by centrifuging through 90% methanol and 100 mM TEAB

Biologically they are the same in terms of result just that they differ in terms of method of absorption

The volume of bacteriostatic water used to reconstitute a lyophilised peptide determines the concentration of the resulting solution, which in turn affects the volume drawn for each dose

Analysis of the mutant proBA operon from a proline-producing strain of Serratia marcescens

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Finally, the purity of the fractions was confirmed by Western blot analysis using cytoplasmic marker protein GAPDH and nuclear marker protein Histone H3(HH3)