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The target protein was eluted by elution buffer A (50 mM sodium phosphate, 150 mM NaCl and 250 mM imidazole, pH 8.0)

308 Intravenous administration of clot busters may be used in severe cases of thrombosis in hospitalized patients

cDNA was synthesized with the iScript cDNA Synthesis Kit (#170-8891, Biorad) and subjected to quantitative real-time PCR using SYBR Green Supermix (#172-5272, Biorad)

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Curr Top Med Chem 1:529539 Valko M, Morris H, Cronin M (2005) Metals, toxicity and oxidative stress