For each qPCR reaction, 25 l of master mix was prepared by adding 12.5 l of the 2 Universal SYBR (ThermoFisher) 5.5 l of RNAse-free water, 1 l ACX primer (0.05 g /ul), 1 l TS primer (0.1 g /l) (Table 1), and 4 l of the protein sample
Behavioral data were analyzed using the KruskalWallis test with Dunns test for multiple comparisons
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A systematic review of the risk of non-alcoholic fatty liver disease in women with polycystic ovary syndrome
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