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K1622) in accordance with the manufacturers instructions 66

Wang D, Luo P, Wang Y, Li W, Wang C, Sun D, Zhang R, Su T, Ma X, Zeng C, Wang H, Ren J, Cao F: Glucagon-like peptide-1 protects against cardiac microvascular injury in diabetes via a cAMP/PKA/Rho-dependent mechanism

For differentiation, 5 10 5 iPSCs per well were seeded in a CELLSTAR6-well plate (Greiner Bio-one) in E6-medium supplemented with 50 ng/mL hbFGF, 1 ng/mL hTGFb1, 50 ng/mL human vascular endothelial growth factor (hVEGF) and human bone morphogenetic protein 4 (hBMP4), 20 ng/mL human stem cell factor (hSCF) (all PeproTech) and 10 M RI and placed on an orbital shaker (OMNI Life Science) at 70 rpm

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