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A seminal cost analysis published in Canadian Family Physician (2001) projected that switching patients from intramuscular B12 injections to oral supplements could save between $2.9 million and $17.6 million depending on the population studied with one Ontario analysis estimating $14.5 million in savings for 110,000 elderly patients

Similarly, long-term monthly fipronil administration has been selected for resistant flea strains, and resistant heartworm-causing Dirofilaria immitis populations have developed, undermining previously effective therapeutic approaches (23)

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Chromosomes were arrested in metaphase, stained with Giemsa R66, and examined microscopically