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A 500 um solution of Dihexa was prepared in acetonitrile and the collected liver microsomes were suspended in a solution of 0.5 mg/ml of 0.1 M Tris buffer at a pH 7.38
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The flow rate is controlled at 0.81.0 mL/min, and the detection wavelength is optimized based on the characteristic absorption of 5amino1mq
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