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After incubating for 30 min at 37 C in the dark, the cells were washed three times with serum-free medium and detected using the Cytoflex flow cytometer (Beckman, USA)

4.1.1 Trans-synaptic transport of -synuclein Misfolded -synuclein aggregates in the ENS, potentially caused by gut inflammation, dysbiosis, or environmental pollutants, can be absorbed by enteric neurons and mucosal epithelial cells

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