Freeze-thaw sensitivity is lower than for a peptide, since there is no secondary structure to lose, but aliquoting still avoids repeated stopper punctures and concentration drift from evaporation
Its scary to think people are injecting this stuff because Joe Rogan said too
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In short, the burps are a downstream consequence of the exact action that makes the drug work
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further 5% -mercaptoethanol was added, and the mixture was again incubated at 95 C for 2 min, and proteins were detected using western blotting