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Cells were lysed by ultrasonication (Sonic Dismembrator Model 500, Fisher Scientific), and the lysate was clarified by centrifugation (35,000g, 30 min)

Patients experiencing hives, facial or throat swelling, wheezing, or difficulty breathing should discontinue use and seek emergency medical care

The binding properties of the expressed receptor are similar to native receptors

I feel very reassured by those data, and I tell patients, If youve maximized the lifestyle changes and you feel like this would be right for you, Im open to that from a breast cancer perspective.