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Plant tissue (0.5 g) was homogenized in 3 mL TCA and centrifuged at 10,000 rpm at 4C for 10 min
thus long-term studies are still needed to confirm that the reward-suppressing action can be maintained over time and with repeated GLP-1 analog administration
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The elution containing the target protein was concentrated to 2 ml for size-exclusion chromatography
They did note a mild rise in gallstone disease (OR 1.30, p = 0.041)
10.1210/jc.2015-4251 99 ValdecantosM
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