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Treatment for 48h with TNF/IFN resulted in diminished expression of Gcg and Mucin 2 ( Muc2 ), a marker for goblet cells, recapitulating in vivo inflammatory conditions ( Figure 3G )

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In addition, a volcano plot and K-means clustering heatmaps were used to show the distribution of significance and fold change of the DEPs between the Sema and HFD groups ( Figures 4C, D )

This is the pattern with the strongest evidence for sustained weight loss