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To label these chimeric receptors at their C-termini with either NLuc or RLuc8, the plasmid was digested with HindIII and XbaI, and the purified fragment was ligated into either Arr3-NLuc or the GLP-1 receptor labeled at the C-terminus with RLuc8 (GLP-1RRLuc8) that had previously been digested with HindIII and XbaI, replacing the Arr3 or GLP-1R open reading frame with that of the chimeric receptor

Finally, we used to model to show how the observed metabolic profile of Down syndrome could arise from trisomy 21 and moderate oxidative stress and how the observed metabolic profile of autism patients could arise from high oxidative stress and increased adenosine concentration

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