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Enzymatic glutathionylation reactions are tightly controlled and highly specific and are considered a major post-translational modification that occurs in response to fluctuations in local redox environments
J Biol Chem 1997
Even though there has been a lot of progress in nanomedicine, the translation of NP-based therapies into clinical practice remains hindered by challenges related to their size, stability, and biodistribution
For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays
Nonetheless, the big fluorescence-bright spots emerged hardly in human normal hepatocytes (L-02)
Like the peptides themselves, however, the combinations of agents have not been well studied
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