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To generate donor plasmids, the left and right homologous arm sequences were amplified from genomic DNA using Phanta Max Super-Fidelity DNA Polymerase (Cat# P505

A multivitamin can offer temporary cover but does not replace whole food

Severe or worsening pain despite proper technique may indicate needle phobia, anxiety-related pain amplification, or skin sensitivity requiring alternative delivery methods

Patients on semaglutide should be counseled on dietary modifications, weight management strategies, and other lifestyle changes that can help mitigate reflux symptoms and enhance the drugs effectiveness in controlling blood glucose levels

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Long-term use of metformin, proton pump inhibitors or H2 blockers , which can reduce B12 absorption over years