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None of these are deal-breakers on their own, but they add up
Peptides were precipitated with cold (4 C) diethyl ether and purified on an Agilent 1200 series High-Performance Liquid Chromatography (HPLC) instrument (1075% HPLC-grade acetonitrile for 20 min at a 2 mL/min flow rate over an Agilent Zorbax C18 column (5 m, 9.4 250 mm) tracked at 220, 254, and 280 nm
It is further reported that APX gene expression is affected by abiotic stresses like salinity, drought, light fluctuations and abcisic acid (ABA) production (Bonifacio et al., 2012
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