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then the supernatant was collected and stored at 20 C till further use

Grx exhibit site-specific distribution with Grx1 being specifically located in cytosol while Grx2 localizes in mitochondria

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Cel Biol 16 (11), 10351044

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Future studies will reveal the applicability of our model, for example, to activate enzymatically inactive glutaredoxins, to alter the sensitivity and responsiveness of glutaredoxin-coupled fluorescent redox sensors, to further address the catalytic mechanism with GSSCys, HEDS and physiological substrates, or to design specific transition-state inhibitors