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Ensure endotoxin testing below 0.5 EU/mg

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Finally, the purity of the fractions was confirmed by Western blot analysis using cytoplasmic marker protein GAPDH and nuclear marker protein Histone H3(HH3)

In contrast to previous studies 18,19,20 , in this work we unequivocally display mixed inhibition mode of KA on both monophenolase and diphenolase activities and undermine the hypothesis regarding copper chelation by KA