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( Epub 2013/09/07 )

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The study design was approved by the Ethics Committee of the first Affiliated Hospital of Sun Yat-Sen University ([2012]248)

Briefly, Nb35 target gene was transformed in BL21 and grown in TB culture medium with 100 g/mL ampicillin, 2 mM MgCl2 and 0.1 % (w/v) glucose at 37C, 180 rpm