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A typical nasal TB-500 protocol might use 500-1000mcg daily during loading phases, dropping to 250-500mcg daily for maintenance

Statements Data availability statement The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author/s

Topical numbing will be applied to the injection site

#### Indications: **Vitamin B12 Deficiency:** Treatment of vitamin B12 deficiency due to malabsorption, dietary insufficiency, or increased physiological needs (e.g., pregnancy, lactation)

Methionine and tryptophan residues in the peptide chain are particularly vulnerable

All supplementation protocols are medically reviewed and monitored to ensure safety, avoid unnecessary excesses, and enhance the overall effectiveness of the treatment program