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Freeze/thaw cycles degrade peptides, so aliquot the reconstituted solution if you plan to run assays over several weeks

However, under a chronic glucolipotoxic environment, persistent hyperglycemia disrupts mitochondrial and endoplasmic reticulum homeostasis to trigger extensive oxidative stress and advanced glycation end-product accumulation, which directly activates various intracellular signaling cascades to initiate RCD pathways (14) ( Table 1 )

It is worthy of further investigation into the underlying processes and their consequences

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& Haeussler, M

Cell Sci