Cells were washed once with PBS, then centrifuge to collect the cells and discard the supernatant
However, the redox regulation within the cells depends on the activity of all ROS producing and eliminating processes (e.g., antioxidant systems) that may vary in different cell types
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Emerging research underscores the potential of molecular hydrogen as a neuroprotective agent, utilizing its selective antioxidant and anti-inflammatory properties
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It is generally indicated as a natural remedy against nervousness, palpitations, anxiety, insomnia, depressive states, menstrual pain, respiratory tract problems, bronchial asthma, and spasms of various kinds.